PART I.

Introduction 11 On Cutting Unprepared Vegetable Tissues 12 On Cutting Unprepared Animal Tissues 13 Preparation of Vegetable Tissues 15 Preparation of Animal Tissues 16 Special Methods of Hardening 19 Cutting Hardened Tissues by Hand 19 Microtome 20 Æther Microtome 24 Section-Knife 24 Imbedding in Paraffine for Microtome 26 Employment of Microtome 29 Staining Agents 31 Carmine Staining 32 Mounting Media 36 Mounting in Glycerine 38 Uses of Freezing Microtome 42 Employment of Freezing Microtome 43 Logwood Staining 47 Absolute Alcohol 50 Clove Oil 51 Canada Balsam 51 Mounting in Balsam 53 Finishing the Slide 55