Section Cutting and Staining A practical introduction to histological methods for students and practitioners

Probably there is nothing more perplexing to a beginner than to decide what apparatus is required. If he consult a price list, it is difficult for him to tell which articles wil...

For the satisfactory examination of tissues it is necessary that they should be “hardened” in certain fluids. The object of this is to give the specimens greater consistence, so...

*Embedding of sections.*--Before sections are made the tissues require to be embedded in some fluid, which will permeate their interstices, and is capable of being rendered firm...

In this method the section whether stained or unstained is placed in a bowl of water, or normal salt solution (p. 53). A clean slide is then introduced into the water at an angl...

Much information may be obtained from unstained sections, and in most cases one section should be examined unstained, but the specimens mounted in this way are so transparent th...

Three methods (two of which are modifications of the first) are employed far more often than any others. By these methods the myelin coating is stained. Tissues must have been h...

It is impossible, within the limits of this work, to attempt any adequate description of the modern methods of bacteriological investigation. Some of these are very lengthy and...

Injection of blood vessels may be performed on small animals, or on individual human organs after removal from the body. The object is to fill the vessels with a coloured fluid...

*Normal histology.*--It cannot be too strongly impressed on the beginner that a thorough mastery of the normal appearances of tissues and organs is absolutely necessary before a...

*Eye.*--Harden the eye of a recently killed bullock, cat, or other animal in formal (p. 23), puncturing the sclerotic in places to allow the hardening fluid to penetrate. In abo...