Chapter 7

The process employed for fixing the lamps is as simple as can be. Each platinum wire is soldered to a piece of copper that surrounds the base of the lamp and that is fixed to the glass with a special cement. These two armatures intertwine, but at a sufficient distance apart to prevent contact. They carry a longitudinal projection and an inflation that fit by hard friction into two copper springs connected electrically with the circuit. It is only necessary to lift the lamp in order to remove it from the support; and the contrary operation is just as easy.--_Le Genie Civil_.

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A NEW REFLECTING GALVANOMETER.

Fig. 1 shows an elevation of the instrument and a horizontal section of the bobbins. Two pairs of bobbins, cc, cc, are so arranged that the axes of each pair are parallel and in the same vertical plane. Each pair is supported by a vertical brass plate, and the two plates make an angle of about 106° with each other, so that the planes containing the axes of the bobbins make an angle of about 74°. Two horseshoe magnets, m m, made of 1/25 inch steel wire, are connected by a very light piece of aluminum and placed at such a distance from each other that, on being suspended, the two branches of each of the magnets shall freely enter the respective bores of the two bobbins fixed upon the same plate, and, when the whole system is in equilibrium and the bobbins free from current, the two branches of each of the magnets shall nearly coincide with the axes of such bores. The magnets are not plane, but are curved so as to form portions of a vertical cylinder whose axis coincides with the direction of the suspension wire, and to which the axes of the bobbins are tangent at their center, approximately to the points where the poles of the magnets are situated.

The needles have been given this form so that their extremities shall not touch the sides of the bore during considerable deflections.

In the instrument which the inventors, Messrs. T. & A. Gray, used in their experiments upon the resistance of glass, the needles were arranged so that their poles of contrary name were opposite.

The system of needles is suspended from the extremity of a screw, p, which passes into a nut, n, movable between two stationary pieces. On revolving the nut, we cause the screw to rise or lower, along with the entire suspended part, without twisting the thread.

The four bobbins are grouped for tension, and have a total resistance of 30,220 ohms. They contain 16,000 feet of No. 50 copper wire, forming 62,939 revolutions, nearly equally divided between the four bobbins. When a current is passing through the bobbins, the poles of one of the horseshoe magnets are attracted toward the interior of the corresponding bobbins, while those of the other are repelled toward the exterior by the two other bobbins. We thus have a couple which tends to cause the system to revolve around the suspension axis. A mirror, which is fixed upon a vertical piece of aluminum, a, gives, in the usual manner, a reflected image upon a scale, thus allowing the deflections to be read. A compensating magnet, M, is supported by a vertical column fixed to the case, above the needles. This magnet may be placed in the different azimuths by means of a tangential screw, t. The extremities of the bobbin wires are connected with three terminals, T, T¹, T², and the instrument may, by a proper arrangement, became differential. These terminals, as well as the communicating wires, are insulated with ebonite.

Thus arranged, the instrument is capable of making a deflection of one division of 1/50 inch upon a scale placed at a distance of a little more than a yard, with the current produced by one daniell of 10 ohms. This is a degree of sensitiveness that cannot be obtained with any of the astatic instruments known up to the present. By regulating the needles properly, a greater degree of sensitiveness may be attained, but then the duration of the needles' oscillation becomes too great. The sensitiveness of the instrument is sufficiently great to allow it to be used in many cases, even with a moderate duration of oscillation.

In their experiments upon the resistance of glass, the inventors employed an instrument that was not arranged for giving great sensitiveness, and one with which resistances of from 10^{4} to 10^{5} megohms could be measured by the use of a pile of 120 daniells.

The instrument can be given another form. The four bobbins may be arranged symmetrically in the same plane, and the two horseshoe magnets be supported by an S-shaped aluminum bar. The latter traverses the plate that supports the bobbins, in such a way that one of the magnets enters one of the bobbins that correspond to it on one side of the plate, and the other on the other side, as shown in Fig. 2. The bobbins are so connected that, when they are traversed by a current, both magnets are at the same time attracted toward the interior or repelled toward the exterior of the bobbins. Such a form of the instrument has the advantage of being more easily constructed, while the regulation of the magnets with respect to the bore of the bobbins is easier.

The chief advantage of the instrument results from the fact that, owing to the arrangement of the magnets and bobbins, a large portion of the wires of the latter is situated very near the poles of the magnets, and in a position very favorable for electro-magnetic action. The instrument presents no difficulties as regards construction, and costs no more than an ordinary one.

We might even arrange a single horseshoe magnet, or an S-shaped one, horizontally, and employ but a single pair of bobbins, and thus have a non-astatic apparatus based upon the same principle. But in astatic instruments it is better to place the magnets in such a way that the two branches shall be in the same vertical plane.

Were the line that joins the two poles vertical, the system would be perfectly astatic in a uniform field, since each magnet in particular would then be perfectly astatic. A pair of horseshoe magnets may thus be regulated in such a way as to form a perfectly astatic system in a uniform field and to preserve an almost invariable zero, this being something that it is very difficult to obtain with the ordinary arrangement of needles, especially when a compensating magnet is used; for, in such a case, one of the needles becomes more or less magnetized, while the other becomes demagnetized, according to the position of the compensating magnet.--_La Lumiere Electrique_.

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HISTOLOGICAL METHODS.

A cat, dog, rabbit, or Guinea pig will furnish parts from which sections can be cut for the study of histology. Whichever animal is selected should be young and well developed. Put it under influence of chloroform, and open into the cavity of the chest; make an incision into the right ventricle, and allow the animal to bleed to death; cut the trachea and inject the lungs with a solution of one and a half drachms of chromic acid in one quart of water, care being taken not to overdistend the lung. Tie the severed end to prevent the escape of the fluid, and carefully remove the lung. It is a difficult thing to do this without rupturing it, but with care and patience it can be done. Place the lungs in a solution of the same strength as used for injecting; after fifteen or twenty hours change it to a fresh solution, and allow it to remain for about a month, and then change it to rectified spirits, in which it may remain until required.

Cut the tongue into several transverse and longitudinal pieces, also the small intestines, and put them into a solution of fifteen and one-half grains chromic acid, thirty grammes bichromate of potash, and three pints of water; change the solution the next day, and let them remain two weeks and then place in spirits. Cut longitudinal and transverse portions of the stomach and large intestines, wash in a weak solution of salt and water, and put them in the same solution as used for the lungs, and treat similarly.

Cut the kidneys longitudinally and transversely, and put them in a solution of six and one-half drachms bichromate of potash, two and one-half drachms sodium sulphate, one quart of water; change the solution the next day, and at the end of four weeks transfer to alcohol. Wash the inner surface of the bladder with salt and water, and after cutting it longitudinally and transversely, put the sections in a solution of three drachms bichromate of potash in a quart of water. Cut the liver into small parts, and place in the same solution as used for the kidneys; change the solution after a day, and let them remain four or five weeks, then change to spirits. The spleen and portions of the thin abdominal muscles may be placed in a solution of three drachms chromic acid to one quart of water, and transferred to alcohol after three or four weeks. Carefully remove an eye and divide it behind the crystalline lens, put the posterior portion in a solution made by dissolving fifteen grs. chromic acid in five drachms water, and slowly adding five and one-half ounces alcohol; change to spirits in two weeks. The lens should be put in the same solution, but should remain a few days longer. Open the head, remove the brain, and place transverse and longitudinal sections of it in spirits for eighteen hours, then transfer to a solution of one drachm chromic acid in a quart of water, and let it remain until hard enough to cut. Place the uterus in a solution of one and one-half drachms chromic acid in one quart of water, change to a new solution the next day, and at the end of a month transfer to alcohol.

The bones from one of the legs should be carefully cleaned of its muscles, cut into several pieces, and placed in a solution of fifteen and one-half grains chromic acid, one-half drachm nitric acid, and six ounces water. Change the fluid frequently until the bones are sufficiently softened, and then change to alcohol.

_Section cutting_ machines for cutting sections can be procured of the dealers, but a very simple and effective one can be easily made if one does not wish to go to the expense of buying an instrument.

A strip of wood twelve or fourteen inches long and about two inches wide has attached to its center a bridge-shaped piece of wood, a, Fig. 1. This is covered with a brass plate, c, pierced with a hole one-half of an inch in diameter. This hole extends through the wood, and is fitted with a piston. Two long narrow inclined planes of nearly equal inclination, b, b, grooved to slide on each other, are placed under the bridge; the lower is to be fastened to the board; the end of the piston rests on the upper one. The object from which we desire to cut a section is placed in the hole, in the piston. If the upper plane be pushed in, the piston will be forced upward, and with it the object. As the inclination of the plane is very gradual, the vertical motion will be very slight as compared with the horizontal.

When the object is raised a little above the brass plate, a keen edged razor, thoroughly wet, is pushed over the hole, cutting the object. This gives the section a smooth surface, and even with the plate; now push the plane forward one-eighth to one-quarter of an inch, and cut again; this will give a thin section of the object. The thickness of the section depends, of course, on the distance the wedge is pushed.

With a little practice, much better sections can be cut by the hand than by any machine; this does not apply of course to large sections. A razor of good steel, with a blade thin and hard, are the most essential points in an instrument for hand cutting. For ordinary purposes it is not necessary to have the blade ground flat on one side, although many prefer it. The knife should always be thoroughly wet, in order that the cut tissue may float over its surface. Water, alcohol or salt and water may be used for this purpose.

_To out a section by hand_, hold the object between the thumb and first two fingers of the left hand, supporting the back of the knife by the forefinger. The knife is to be held firmly in the right hand, and in cutting should never be pushed, but drawn from heel to point obliquely through the tissue. The section should be removed from the knife by a camel's hair brush.

When the object is too small to hold, it is usually _embedded_ in some convenient substance. A carrot is sometimes very useful for this purpose. A hole rather smaller than the object is cut out of the middle. Put whatever is to be cut into this, and cut a thin section of the whole. The carrot does not cling to either the knife or the section, and the knife is wetted at every slice by it.

Paraffin is the agent usually employed for embedding purposes. Melt it, and add a little lard to soften it; the addition of a little clove oil renders it less adhesive.

Melt the paraffin at as low a temperature as possible, and pour it into a paper cone. Dip the object into this and remove immediately; as soon as the layer of paraffin surrounding it becomes hardened, replace it in the paraffin; this prevents overheating the tissues.

Where the tissues are too soft to be cut, they may be soaked in a solution of gum arabic and dried; in this condition they can be readily cut, after which the gum can be dissolved off. This is an extremely useful method for cutting the lung or other organs where an interstitial support is needed. For a very thin object, a cork fitting any kind of a tube is to be split, and the object placed between the two parts; the cork is then thrust into the tube, and a sufficient degree of firmness will be obtained to allow cutting. The sections should always be manipulated with camel's hair brushes.

Much practice will be required before dexterity is attained.

_Methods of preserving the tissues_.--All water must be removed from the tissue, either by drying or by immersing it in rectified spirits, and then in absolute alcohol, and the alcohol driven off by floating it upon oil of clove or turpentine. The substances used to preserve the tissues are Canada balsam, Dammar balsam, glycerine, Farrant's solution, potassium acetate, spirits, naphtha, and creosote.

The section is to be floated on to the slide or placed in position with a camel's hair brush. It should be spread out, and then examined under the microscope for the purpose of improving its position if necessary, or of removing any foreign particles. A drop of the preserving medium is then placed upon it, and another placed on the cover and allowed to spread out. The cover is then taken by a pair of pincers and inverted over the object, and one edge brought to touch the slide at one part of its margin. The cover is then gently lowered, and the whole space beneath the cover filled and the tissue completely saturated. If air bubbles show themselves, raise the cover at one corner and deposit a further quantity of the medium.

The slide should be set aside for a few days. First, the excess of the medium must be removed; if it is glycerine, much of it can be removed by a piece of blotting paper, but the cover must not be touched, for it is easily displaced; that near the cover can be replaced by a camel's hair brush. A narrow ring of glycerine jelly should be placed around the edge of the cover, to fix it before the cement is applied. When this has set, a narrow strip of cement is to be put on, just slightly overlapping the edge of the cover and outside the margin of the jelly. Until it has been perfectly secured, a slide carrying glycerine must never be placed in an inclined position, as its cover will slide off.

_Preservative media_.--Canada balsam may be prepared as follows: Place some pure Canada balsam in a saucer, and cover with paper to exclude dust; dry it in an oven at a temperature of 150°; when it cools, it will become hard and crystalline. Dissolve this in benzole, and use in the same way as glycerine.

Dammar is now used as a substitute for Canada balsam. By its use the tissues are rendered more transparent. To prepare it, dissolve one-half ounce of Dammar rosin and one-half ounce of gum mastic in three ounces of benzole, and filter. This may be used to mount unsoftened bone and tooth, hair, brain, and spinal column, and most tissues that have been hardened in alcohol or chromic acid, which require to have their transparency increased.

Glycerine is not adapted for white fibrous tissue or blood vessels, unless they have been hardened in chromic acid, as it causes the white fibers to swell up and lose their normal features. Sections of liver, lung, skin, and alimentary canal show better in glycerine unless they have been stained.

Farrant's solution may be substituted for glycerine in many instances, because of its feebler tendency to render the tissues transparent. It consists of equal parts of gum arabic, glycerine, and a saturated solution of arsenious acid. In mounting preparations with this medium, the covered object should be allowed to lie a day before the varnish is applied, so that the cover may be fixed, and thereby prevented from being displaced. Rectified spirits may be used for mounting softened bone and tooth, and naphtha and creosote are useful for preserving urinary casts.

When the section is mounted in Canada or Dammar balsam, no cement is required, but for all other preservative media the margin of the cover must be covered with cement. To do this, dry the edges of the cover thoroughly with bibulous paper, and paint a layer of gold size, allowing it to overlap the cover an eighth or sixteenth of an inch, then cover this with white zinc cement.

_Preparation for mounting the different tissues_.--To obtain a section of bone or tooth requires a grinding down of the tissue until it is so thin as to be transparent. A section should first be cut as thin as possible by a fine saw. It should be attached by the flattest side to a piece of glass, and then ground down by a grindstone or by very fine emery, on a perfectly flat piece of lead. When sufficiently thin and transparent, mount in rectified spirits or Dammar. Sections of the tongue may be made by embedding in paraffin, and mounted in Farrant's solution or glycerine.

Sections of the stomach may also be made by embedding in paraffin, but better ones can be made by freezing. Farrant's solution makes a good mounting.

The intestines also give a better section from freezing than by embedding, as the paraffin injures the villi; mount in the same medium as the stomach.

The liver may be embedded in paraffin, and the section mounted in Farrant's solution or glycerine. The kidney may be treated in the same way. The cornea of the eye can be readily cut by embedding in paraffin, and the section may be mounted in Farrant's solution. The crystalline lens and retina may be treated similarly.

The brain and spinal cord should be embedded in paraffin or a carrot, and the section mounted in Dammar. Sections of the uterus and ovaries are best mounted in glycerine or Dammar. Sections of lung maybe made by embedding in gum or by freezing, and mounted in Farrant's solution.

Every slide should be of uniform size, and labeled. The usual size is 3×1 inches, and should be of a good quality of glass, free from scratches or air holes. They may be labeled either by writing with a diamond, or a small piece of paper affixed to one end, on which is written what is required.

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LIFE HISTORY OF A NEW SEPTIC ORGANISM.

At a recent meeting in London, of the Royal Miscroscopical Society, Dr. Dallinger gave his annual address to what was probably the largest gathering of Fellows ever assembled on a similar occasion. After briefly referring to the increased interest lately manifested in the study of minute organisms, and recalling the characteristics of the doctrines of abiogenesis and biogenesis, he passed rapidly in review the results of the observations of Tyndall, Huxley, and Pasteur as bearing upon these questions, and called attention to the observations of Buchner as to the transformation of _Bacillus anthracis_ and _Bacillus subtilis_, and _vice versa_, and referred with approval to Dr. Klein's criticisms thereon. Having spoken of the desirability of careful and continuous study of this class of organisms, and the importance of endeavoring to establish the relation of the pathogenic form to the whole group, he said he should be better able to deal with the subject by recording a few ascertained facts rather than by making a more extended review, and he therefore devoted the main part of his address to a description of "the life history of a septic organism hitherto unknown to science." In his observations of this form--extending over four years--he had the advantage of the highest quality of homogeneous lenses obtainable, ranging from one-tenth to one-fiftieth of an inch, his chief reliance being placed upon a very perfect one thirty-fifth of an inch; and from the continuous nature of the observations as well as the circumstances under which they were carried on, dry lenses had for the most part to be employed. Having in his possession a maceration of cod-fish in a fluid obtained from boiled rabbits, he found at the bottom of it, when in an almost exhausted condition, a precipitate forming a slightly viscid mass, to which his attention was particularly directed. It was seen to contain a vast number of _Bacterium termo_, but on examination with a one-tenth inch objective showed that it also contained a comparatively small number of intensely active organisms--one being discovered in about eight or ten drops of the sediment. These measured 1-10,000 of an inch in length by 1-19,500 of an inch in breadth. The fluid had originally been kept at a temperature of 90° to 95° F., and it was noticed that, when placed upon a cold stage under the microscope, the movements of the organisms became, gradually slower, until at last they entirely ceased; the necessity, therefore, arose for the use of a warm stage, and the very ingenious contrivance by which a continuous and even temperature was maintained within the one-tenth of a degree was exhibited. The greatest difficulty in the matter was, however, experienced in obtaining specimens for observation, in order to be able to trace them from their earliest to their latest stage. The President then explained, by means of an admirable series of illustrations projected upon a screen by the oxyhydrogen lantern, the life history of the organism to which he had referred, exhibiting it first as a translucent, elliptic, spindle-shaped body, with six long and delicate flagella, the various positions in which the five specimens were drawn giving a very good idea of its peculiar porpoiselike movements.