Part 14
These require little or no preparation. The first object is to procure them, the second, to render them visible by the microscope. A few observations, however, may be of use. Many drops of water may be examined before any can be found; so that if the observer be too hasty, he may be easily disappointed, though other parts of the same water may be fully peopled by them.
The surface of infused liquors is generally covered with a thin pellicle, which is easily broken, but acquires thickness by standing; the greatest number of animalcula are generally to be found in this superficial film.
In some cases it is necessary to dilute the infusions; but this is always to be done with distilled water, and that water should be examined in the microscope before it is made use of: the neglect of this precaution has been a source of many errors.
Animalcula are in general better observed when the water is a little evaporated, as the eye is not confused, nor the attention diverted by a few objects. To separate one or two animalcula from the rest, place a small drop of water on the glass near that of the infusion; make a small neck or gutter between the two drops with a pin, which will join them together; then the instant you perceive that an animalculum has traversed the neck or gutter, and entered the drop, cut off the communication between the two drops.
To procure the eels in paste, boil a little flower and water, till it becomes of a moderate consistence; expose it to the air in an open vessel, and beat it together from time to time, to prevent the surface thereof from growing hard or mouldy; after a few days, especially in summer time, it will turn sower, then if it be examined with attention, you will find myriads of eels on the surface.
To preserve these eels all the year, you must keep the surface of the paste moist, by putting a little water or fresh paste from time to time to the other. Mr. Baker advises a drop or two of vinegar to be put into the paste now and then. The continual motion of the eels, while the surface is moist, will prevent the paste getting mouldy. Apply them to the microscope upon a slip of flat glass, first putting on it a drop of water, taken up by the head of a pin, for them to swim in.
To make an infusion of pepper. Bruise as much common black pepper as will cover the bottom of an open jar, and lay it thereon about half an inch thick; pour as much soft water in the vessel as will rise about an inch above the pepper. The pepper and water are then to be well shaken together; after which they must not be stirred, but be left exposed to the air for a few days, when a thin pellicle will be formed on the surface of the water, containing millions of animalcula.
The observer should be careful not to form a judgment of the nature, the use, and the operations of small animalcula, from ideas which he has acquired by considering the properties of larger animals: for, by the assistance of glasses, we are introduced as it were into a new world, and become acquainted not only with a few unknown animals, but with numerous species thereof, which are so singular in their formation and habits, that without the clearest proofs even their existence would not be credited; and while they afford fresh instances of the Creator’s power, they also give further proofs of the limits and weakness of the human understanding.
DIRECTIONS FOR FINDING, FEEDING, AND PRESERVING THE POLYPES.
These little animals are to be found upon all sorts of aquatic plants, upon branches of trees, pieces of board, rotten leaves, stones, and other substances that lie in the water; they are also to be met with upon the bodies of several aquatic animals, as on the water-snail, on several species of the monoculus, &c. they generally fix themselves to these by their tail, so that it is a very good method when you are in search of the polypes, to take up a great many of these substances, and put them in a glass full of water. If there be any polypes adhering to these, you will soon perceive them stretching out their arms, especially if the glass be suffered to be at rest for a while; for the polypes, which contract themselves on being first taken out of the water, will soon extend again when they are at rest.
They are to be sought for in the corners of ditches, puddles, and ponds, being frequently driven into these with the pieces of wood or leaves to which they have attached themselves. You may, therefore, search for them in vain at one period, in a place where at another they will be found in abundance. They are more easily perceived in a ditch when the sun shines on the bottom, than at another time. In winter they are seldom to be met with; about the month of May they begin to appear and increase.
They are generally to be found in waters which move gently; for neither a rapid stream, nor stagnant waters ever abound with them. As they are always fixed to some substance by their tails, and are very rarely loose in the water, taking up water only can signify but little; a circumstance which has probably been the cause of much disappointment to those who have searched for them.
The green polypes are usually about half an inch long when stretched out; those of the second and third sort are between three quarters of an inch and an inch in length, though some are to be found at times which are an inch and a half long.
Heat and cold has the same effect upon these little creatures, that it has upon those of a larger size. They are animated and enlivened by heat, whereas cold renders them faint and languid; they should therefore be kept in such a degree of heat, that the water may not be below temperate.
It is convenient for many experiments to suspend a polype from the surface of the water. To effect this, take a hair pencil in one hand, and hold a pointed quill in the other; with the pencil loosen the polype from the receiver in which it is kept, and gradually raise it near the top of the water, so that the anterior end may be next the point of the pencil; then lift it out of the water, and keep it so for a minute; after which, thrust the point of the pencil, together with the anterior end, by little and little under water, until no more than about the twentieth part of an inch of the polype’s tail remains above its surface; at this instant, with the pointed quill remove that part of the polype from the pencil which is already in the water, at the same time blowing against the polype, by which it will be loosened, and remain out of the water.
When the polypes were first discovered, Mr. Trembley had some difficulty to find out the food which was proper for them; but he soon discovered, that a small species of the millepede answered the purpose very well: the pulices aquatices have also been recommended. The small red worms, which are to be found on the mud-banks of the Thames, particularly near the shores, answer the purpose also, they are easily found when the tide is out, when they rise in such swarms on the surface of the mud, that it appears of a red colour. These worms are an excellent food for the polype. If a sufficient quantity be gathered in November, and put into a large glass full of water, with three or four inches of earth at the bottom, you will have a supply for the polypes all the winter. They may also be fed with common worms, with the larva of gnats and other insects, and even with butcher’s meat, &c. if it be cut small enough.
River, or any soft water, agrees with them; but that which is hard and sharp prevents their thriving, and generally kills them in a few days. The worms with which they are fed should be always cleansed before you feed the polypes with them.
The polypes are commonly infested with little lice; from these it is necessary to free them, in order to preserve your polypes in a good state of health. They may be cleansed from the lice by rubbing them with a hair pencil; this cannot be easily done, unless they adhere to some substance: so that if they are suspended from the surface of the water, you must endeavour to get them to fix themselves to a piece of packthread; when they are fastened thereto, you may then rub them with a hair pencil, without loosening them from the thread.
The lice which torment the polype are not only very numerous, but they are also very large proportionably to its size: they may be said to be nearly as large with respect to them, as a common beetle is to us. If not rubbed off, they soon cover their bodies, and in a little time totally destroy them.
To preserve the polypes in health, it is also necessary often to change the water they are kept in, and particularly after they have done eating; it is not sufficient to pour the water off, all the polypes should be taken out, and the bottom and sides of the vessel rubbed from the slimy sediment adhering thereto; this is caused by their fæces, and is fatal to them if not cleaned away. The fæces often occasion a species of mortification, which daily increases; its progress may be stopped by cutting off the diseased part. To take them out, first loosen their tails from the sides or bottom of the glass; then take them up one by one, with a quill cut in the shape of a scoop, and place them in another glass with clean water; if they cling to the quill, let it remain a minute or two in the water, and they will soon disengage themselves.
They are preserved best in large glasses that hold three or four quarts of water; for in a glass of this size the water need not be renewed so often, particularly, if the fæces are taken out from time to time with the feathered end of a pen, to which they readily adhere; and further, the trouble of feeding each individual is in some measure saved, as you need only throw in a parcel of worms, and let the polypes divide them for themselves.
To observe with accuracy the various habitudes, positions, &c. of this little animal, it will be necessary to place some of them in narrow cylindrical glasses; then, by means of the microscope, Fig. 3. Plate VI. you may observe them exerting all their actions of life with ease and convenience; the facility with which the lens of the fore-mentioned microscope may be moved and placed in any direction, renders it a most convenient instrument for examining any object that requires to be viewed in water.
It is also very proper to dry some of them, and place them between talcs in a slider; this, however requires some dexterity and a little practice; though, when executed with success, it fully rewards the pains of the observer. Choose a proper polype, and put it into a small concave lens, with a drop of water; when it is extended, and the tail fixed, pour off a little of the water, and then plunge it with the concave into some spirit of wine contained in the bowl of a large spoon; by this it is instantly killed, the arms and body contracting more or less; rub it gently while in the spirit with a small hair pencil, to cleanse it from the lice.
The difficulty now begins; for the parts of the polype, on being taken out of the spirit, immediately cling together, so that it is not practicable to extend the body, and separate the arms on the talc, without tearing them to pieces; therefore the only method is, to adjust them upon the talc while in the spirit: this may be done by slipping the talc under the body of the polype, while it lies in the spirit, and displaying its arms thereon by the small hair pencil and a pair of nippers; then lift the talc, with the polype upon it, out of the spirit; take hold of it with the nippers in the left hand, dip the pencil in the spirit with the right hand, and therewith dispose of the several parts, that they may lie in a convenient manner, at the same time brushing away any lice that may be seen upon the talc; now let it dry, which it does in a little time, and place the talc carefully in the hole of the slider. To prevent the upper talc and ring pressing on the polype, you must cut three pieces of cork, about the bigness of a pin’s head, and the depth of the polype, and fix them by gum in a triangular position, partly on the edges of the said talc, and partly to the sides of the ivory hole itself; the upper talc may then be laid on these corks, and pressed down by the ring as usual.[45]
[45] Baker on the Polypes.
OF VEGETABLES.
It were to be wished a satisfactory account could here be given of all the preparations which are requisite to fit for the microscope the objects of the vegetable kingdom. Dr. Hill is the only writer who has handled this subject. I shall, therefore, extract from his “Treatise on the Construction of Timber,” what he has said; this, together with the improvements I have made on the cutting engine, will enable the reader to pursue the subject and extend it further, both for his own pleasure, and the advantage of the public.
THE MANNER OF OBTAINING THE PARTS OF A SHOOT SEPARATE.
In the beginning of April, take a quantity of young branches from the scarlet oak, and other trees. These are first cut into lengths, of the growth of different seasons; and then part is left entire, part split, and the rest quartered. In this state they are put into a wicker basket, with large openings, or of loose work, and a heavy stone is put in with them; a rope is tied to the handle of the basket, and it is thrown into a brook of running water: at times it is taken up, and exposed a little to the air; it is frequently shook about under water, to wash off filth; and once in ten days the sticks are examined.
By degrees the parts loosen from one another, and by gentle rubbing in a bason of water just warmed, they will be so far separated, that a pencil brush will perfect the business, and afford pieces of various sizes, pure, distinct, and clean. One part will in this way separate at one time, and another, at another; but by turning the sticks to the water, and repeating the operation, in the course of four or five weeks every part may be obtained distinct. They are best examined immediately; but if any one wish to preserve them for repeated inquiries, it may be done in this manner: dissolve half an ounce of alum in two quarts of water; drop the pieces thus separated, for a few moments, into this solution, then dry them upon paper, and put them up in vials of spirit of wine, no other fluid being so well adapted to preserve these tender bodies.
TO PREPARE THE RIND FOR OBSERVATION.
As the vessels of the rind are of different diameters in various trees, though their construction and that of the blebs is perfectly the same in all, it will be best to choose for this purpose the rind of a tree wherein they are largest. The rind of the ash-leaved maple is finely suited. A piece of this may be obtained of two inches long, and will very successfully answer the intention. Such a piece being prepared without alum or spirit, but dried from the water in which it had been macerated, it is to be impregnated with lead in the following manner, to shew the apertures by their colour.
Dissolve one drachm of sugar of lead in an ounce and an half of water; filter this through paper, and pour it into a tea-cup. Clip off a thin slice of what was the lower end of the piece of rind as it grew on the tree, and plunge it near an inch deep into the liquor; keep it upright between two pieces of stick, so that one half or more may be above the water; whelm a wine-and-water glass over the tea-cup, and set the whole in a warm place. When it has stood two days, take it out, clip off all that part which was in the liquor, and throw it away.
The circumstances here mentioned, trivial as they may seem, must be attended to: the operation will not succeed, even if the covering-glass be omitted; it keeps a moist atmosphere about the rind, and makes its vessels supple.
While this is standing, put into a bason two ounces of quick lime, and an ounce of orpiment; pour upon them a pint and an half of boiling water; stir the whole together, and when it has stood a day and a night, it will be fit for use. This is the “liquor probatorius vini” of some of the German chymists; it discovers lead when wines are adulterated with it, and will shew it any where.
Put a little of this liquor in a tea-cup, and plunge the piece of rind half way into it.
In the former part of this experiment, the vessels of the rind have been filled with a solution of lead, that makes of itself no visible alteration in them; but this colourless impregnation, when the orpiment lixivium gets to it, becomes of a deep brown; the vessels themselves appear somewhat the darker for it; but these dots, which are real openings, are now plainly seen to be such, the colour being perfectly visible in them, and much darker than in the vessels. This object must be always viewed dry.
If a piece of the rind, thus impregnated, be gently rubbed between the fingers, till the parts are separated, we shall be able in one place or other, to get a view of the vessels all round, and of the films which form the blebs between them.
Every part of the rind, and every coat of it, even the interstitial place between its innermost coat and bark, are filled with a fine fluid. The very course and progress of the fluid may be shewn in this part, even by an easy preparation; only that different rinds must be sought for this purpose, the vessels in some being larger than in others. Repeated trials have shewn me that the whole progress may be easily marked in the three following kinds, with only a tincture of cochineal.
Put half an ounce of cochineal, in powder, into half a pint of spirit of wine; set it in a warm place, and shake it often for four days; then filter off the clear tincture. Put an inch depth of this into a cup, and set upright in it pieces of the rind of ash, white willow, and ozier, prepared as has been directed, by maceration in water; for in that way one trouble serves for an hundred kinds. Let an inch of the rinds also stand up out of the tincture. After twenty-four hours take them out, clip off the part which was immersed in the fluid, and save the rest for observation.
TO PREPARE THE BLEA.
Cut the pieces in a fit season, either just before the first leaves of Spring, or in the Midsummer shooting time. Then we see all the wonders of the structure; the thousands of mouths which open throughout the course of these innumerable vessels, to pour their fluid into the interstitial matter.
These vessels, which are in nature cisterns of sap for the feeding the growth of the whole tree, are so large, that they are capable of being filled with coloured wax, in the manner of the vessels in anatomical injections; and this way they present pleasing objects for the microscope, and afford excellent opportunities of tracing their course and structure.
A METHOD OF FILLING THE SAP VESSELS OF PLANTS.
A great many shoots of the scarlet and other oaks are to be taken off in the Spring; they must be cut into pieces of about two inches in length, and immediately from the cutting they must drop into some warm rain water: in this they are to stand twenty-four hours, and then be boiled a little. When taken out, they are to be tied on strings, and hung up in a place where the air passes freely, but the sun does not shine. When they are perfectly dry, a large quantity of green wax, such as is used for the seals of law deeds, is to be gently melted in an earthen pipkin set in water; the water to be heated and kept boiling. As soon as the wax runs, the sticks are to be put in, and they are frequently to be stirred about. They must be kept in this state about an hour, and then the pipkin is to be taken out of the water, and set upon a naked fire, where it is to be kept with the wax boiling for two or three hours; fresh supplies of the same wax being added from time to time.
After this it is to be removed from the fire, and the sticks immediately taken out with a pair of nippers; when they are cold, the rough wax about them is to be broken off. Both ends of each stick are to be cut off half an inch long, and thrown away, and the middle pieces saved. These are then to be cut in smaller lengths, smoothed at the ends with a fine chissel, and many of them split in various thicknesses.
Thus are obtained preparations, not only of great use, but of wonderful beauty. Many trees this way afford handsome objects as well as the oak; and in some, where the sap vessels are few, large, and distinct, the split pieces resemble striped satins, in a way scarce to be credited. It is in such that the outer coats of these vessels are most happily of all to be examined.
THE METHOD OF PREPARING SALTS AND SALINE SUBSTANCES, FOR THE VIEWING THEIR CONFIGURATIONS.
Dissolve the subject to be examined in no larger a quantity of river or rain water than is sufficient to saturate it; if it be a body easily dissoluble, make use of cold water, otherwise make the water warm or hot, or even boiling, according as you find it necessary. After it is perfectly dissolved, let it rest for some hours, till, if over-charged, the redundant saline particles are precipitated, and settle at the bottom, or shoot into crystals; by which means you are most likely to have a solution of the same strength at one time as at another; that is, a solution fully charged with as much as it can hold up, and no more; and by these precautions the configurations appear alike, how often soever tried: whereas, if the water be less saturated, the proportions, at different times, will be subject to more uncertainty; and if it be examined before such separation and precipitation of the redundant salts, little more will be seen than a confused mass of crystals.
The solution being thus prepared, take up a drop of it with a goose quill, cut in fashion of a scoop, and place it on a flat slip of glass, of about three quarters of an inch in width, and between three and four inches long, spreading it on the glass with the quill, in either a round or oval figure, till it appears a quarter of an inch or more in diameter, and so shallow as to rise very little above the surface of the glass. When it is so disposed, hold it as level as you can over the clear part of a fire that is not too fierce, or over the flame of a candle, at a distance proportionable to the degree of heat it requires, which experience only can direct, and watch it very carefully till you discover the saline particles beginning to gather and look white, or of some other colour, at the extremities of the edges; then having adjusted the microscope before-hand for its reception, armed with the fourth glass, which is the fittest for most of these experiments, place it under your eye, and bring it exactly to the focus of the magnifier; and after running over the whole drop, fix your attention on that side where you observe any increase or pushing forwards of crystalline matter from the circumference towards the center.
This motion is extremely slow at the beginning, unless the drop has been over-heated, but quickens as the water evaporates, and in many kinds, towards the conclusion, produces configurations with a swiftness inconceivable, composed of an infinity of parts, which are adjusted to each other with an elegance, regularity, and order, beyond what the exactest pencil in the world, guided by the ruler and compass, can ever equal, or the most luxurious imagination fancy.
When action once begins, the eye cannot be taken off, even for a moment, without losing something worth observation; for the figures alter every instant, till the whole process is over; and in many sorts, after all seems at an end, new forms arise, different entirely from any that appeared before, and which probably are owing to some small quantity of salt of another kind, which the other separates from, and leaves to act after itself has done; and in some subjects three or four different sorts are observable, few or none being simple and homogeneous.