Part 13

Sometimes he put into water the delicate viscera of the insects he had suffocated; and then shaking them gently he procured himself an opportunity of examining them, especially the air vessels, which by these means he could separate from all the other parts whole and intire, to the great admiration of all those who beheld them; as these vessels are not to be distinctly seen in any other manner, or indeed seen at all without damaging them, he often made use of water, injected by a syringe, to cleanse thoroughly the internal parts, then blew them up with air and dried them, and thus rendered them durable, and fit for examination at a proper opportunity. Sometimes he has examined with the greatest success, and made the most important discoveries in insects that he had preserved in balsam, and kept for years together in that condition. Again, he has frequently made punctures in other insects with a very fine needle, and after squeezing out all their moisture through the holes made in this manner, he filled them with air, by means of very slender glass tubes, then dried them in the shade, and last of all anointed them with oil of spike, in which a little rosin had been dissolved; by which process they retained their proper forms a long time. He had a singular secret, whereby he could so preserve the nerves of insects, that they used to continue as limber and perspicuous as ever they had been.

He used to make a small puncture or incision in the tail of worms, and after having gently and with great patience squeezed out all their humours, and great part of their viscera, he then injected them with wax, so as to give and continue to them all the appearance of healthy vigorous living creatures. He discovered that the fat of all insects was perfectly dissoluble in oil of turpentine; thus he was enabled to shew the viscera plainly; only after this dissolution he used to cleanse and wash them well and often in clean water. He frequently spent whole days in thus cleansing a single caterpillar of its fat, in order to discover the true construction of this insect’s heart. His singular sagacity in stripping off the skin of caterpillars that were upon the point of spinning their cones, deserves particular notice. This he effected by letting them drop by their threads into scalding water, and suddenly withdrawing them; for, by these means the epidermis peeled off very easily; and when this was done, he put them into distilled vinegar and spirit of wine, mixed together in equal portions, which, by giving a proper firmness to the parts, gave him an opportunity of separating them with very little trouble from the exuviæ, or skins, without any danger to the parts; so that by this contrivance, the nymph could be shewn to be wrapped up in the caterpillar and the butterfly in the nymph. Those who look into the works of Swammerdam, will be abundantly gratified, whether they consider his astonishing labour and unremitted ardour in these pursuits, or his wonderful devotion and piety. On one hand, his genius urged him to examine the miracles of the great Creator in his natural, productions; whilst, on the other, the love of that same all-perfect Being rooted in his mind struggled hard to persuade him that God alone, and not the creatures, were worthy of his researches, love, and attention.

M. Lyonet always drowned first those insects he intended to anatomize, as by these means he was enabled to preserve both the softness and transparency of the parts. If the insect, &c. be very small, for instance one-tenth of an inch, or a little more in length, it should be dissected in water, on a glass which is a little concave; if, after a few days, there be any fear that the insect will putrefy, it should be placed in weak spirit of wine, instead of water. In order to fix the little creature, it must be suffered to dry, and then be fastened by a piece of soft wax; after which it may be again covered with water.

Larger objects require a different process; they should be placed in a small trough of thin wood; the bottom of a common chip box will answer very well, by surrounding the edge of it with soft wax, to keep in the water or spirit of wine. The insect is then to be opened, and if the parts be soft, like those of a caterpillar, they should be turned back and fixed to the trough by small pins; the pins are to be set by a pair of small nippers, the skin being stretched at the same instant by another pair of finer forceps; the insect must then be placed in water, and dissected therein, and after two or three days it should be covered with spirit of wine, which should be renewed occasionally; by these means the subject is preserved in perfection, and its parts may be gradually unfolded, without any other change being perceived than that the soft elastic parts become stiff and opake, and some others lose their colour.

M. Lyonet used the following instruments in his curious dissection of the caterpillar of the cossus. As small a pair of scissars as could be made, the arms long and fine; a small and sharp knife, the end brought to a point; a pair of forceps, the ends of which had been so adjusted, that they would easily lay hold of a spider’s thread or a grain of sand. But the most useful instruments were two fine steel needles, fixed in small wooden handles, about 2³⁄₄ of an inch in length.

An observation of Dr. Hooke’s may be very useful if attended to, for fixing objects intended to be delineated by the microscope. He found no creature more troublesome to draw than the ant or pismire, not being able to get the body quite in a natural posture. If, when alive, its feet were fettered with wax or glue, it would so twist and twine its body, that it was impossible any way to get a good view of it; if it was killed, the body was so small, that the shape was often spoiled before it could be examined. It is the nature of many minute bodies, when their life is destroyed, for the parts to shrivel up immediately; this is very observable in many small plants, as well as in insects; the surface of these small bodies, if porous, being affected by almost every change of the air, and this is particularly the case with the ant. But if the little creature be dropped in well rectified spirit or wine, it is immediately killed; and when taken out, the spirit of wine evaporates, leaving the animal dry and in its natural posture, or at least so constituted, that you may easily place it with a pin in what posture you please.[42]

[42] Hooke’s Micrographia, p. 203.

Having thus given a general account of the methods used by Swammerdam and Lyonet, in their examination and dissection of insects, we shall proceed to shew how to prepare several of their parts for the microscope, beginning with the WINGS. Many of these are so transparent and clear, as to require no previous preparation; but the under wings of those that are covered with elytra, or crustaceous cases, being constantly folded up when at rest, they must be unfolded before they can be examined by the microscope; for this purpose a considerable share of dexterity and some patience is necessary, for the natural spring of the wings is so strong, that they immediately fold themselves again, except they are carefully prevented.

One of the most curious and beautiful wings of this kind, is that of the FORFICULA AURICULARIA, or EARWIG, of which we have given a drawing, Plate XIV. Fig. 1, represents it considerably magnified, and Fig. 2, the same object of its natural size. When expanded, it is a tolerably large wing, yet folds up under a case not one-eighth part of its size. It is very difficult to unfold these wings, on account of their curious texture. They are best opened immediately after the insect is killed. Hold the earwig by the thorax, between the finger and thumb; then with a blunt-pointed pin endeavour gently to open the wing by spreading it over the fore-finger, gradually sliding at the same time the thumb over it. When fully expanded, separate it from the insect by a sharp knife, or a pair of scissars. The wing should be pressed for some time between the thumb and finger before it be removed; it may then be placed between two pieces of paper, and again pressed for at least an hour; after which it may be put between the talcs without any danger of folding up again.

The wings of the NOTONECTA, or BOAT-FLY, and other water insects, as well as most species of the grylli, require equal care and delicacy with that of the earwig to display them properly.

The wings of BUTTERFLIES and MOTHS are covered with very minute scales or feathers, that afford a beautiful object for the microscope; near the shoulder, the thorax, the middle of the wing, and the fringes of the wings, they are generally intermixed with hair. The scales of one part, also, often differ in shape from those of another; they may be first scraped off or loosened from the wing with a knife, and then brushed into a piece of paper with a camel’s hair pencil; the scales may be separated from the hairs with the assistance of a common magnifying glass.

The proboscis of insects, as of the CULEX or GNAT, the TABANUS or BREEZE-FLY, &c. requires much attention and considerable care to be dissected properly for the microscope; and many must be prepared before the observer desides upon the situation and shape of the parts; he will often also be able to unfold in one specimen some parts that he can scarce discover in another. It is well known that the COLLECTOR OF THE BEE forms a most beautiful object; a figure of it is given in plate XIII. Fig. 3, shews it greatly magnified, and Fig. 4, of the natural size. In it is displayed a most wonderful mechanism, admirably adapted to collect and extract the various sweets from flowers, &c. To prepare this, it should first be carefully washed with spirit of turpentine, by which means it will be freed from the unctuous and melliferous particles which usually adhere to it; when dry, it must be again washed with a camel’s hair pencil, to disengage and bring forward the small hairs which form one part of its microscopic beauty.

The case which encloses THE STING OF THE BEE, the wasp, and the hornet, are so hard, that it is very difficult to extract them without breaking or otherwise injuring them. It will be found, perhaps, the best way to soak the case, and the rest of the apparatus for some time in spirit of wine or turpentine, then lay it on a piece of clean paper, and with a blunt knife draw out the sting, holding the sheath by the nail of the finger, or by any blunt instrument; great care is requisite to preserve the feelers, which when cleaned add much to the beauty of the object.

The EYES OF THE LIBELLULA or DRAGON-FLY, and different flies, of the LOBSTER, &c. are first to be cleaned from the blood and other extraneous matter; they should then be soaked in water for some days, after which you may separate one or two skins from the eye, which, if they remain, render it too opake and confused; some care is, however requisite in this separation, otherwise the skin may be made too thin, so as not to enable you to form an accurate idea of its organization.

The EXUVIÆ or CAST-OFF OF SKINS of insects are in general very pleasing objects, and require but little preparation. If they be curled or bent up, keep them in a moist atmosphere for a few hours, and they will soon become so relaxed that you may extend them with ease to their natural positions. The steam of warm water answers the purpose very well.

The BEARD OF THE LEPAS ANATIFERA or BARNACLE is to be soaked in clean soft water, and frequently brushed, while wet, with a camel’s hair pencil; it may then be left to dry; after which it must be again brushed with a dry pencil, to disengage and separate the hairs, which are apt to adhere together. A picture of this object is represented in plate XIII. Fig. 1, magnified; Fig. 2, natural size.

To view the MUSCULAR FIBRES, take a very thin piece of dried flesh, lay it upon a slip of glass, and moisten it with warm water; when this is evaporated, the vessels will appear plain and more visible, and by repeated macerations the parts may be further disengaged.

To examine FAT, BRAINS, and other similar substances, we are advised by Dr. Hooke to render the surface smooth, by pressing it between two thin plates of flat glass, by which the substance will be made much thinner and more transparent; otherwise, the parts lying thick one upon the other, it appears confused and indistinct.

Some substances are, however, so organized, that if their peculiar form be altered, the parts we wish to discover are destroyed; such as nerves, tendons, muscular fibres, pith of wood, &c. Many of these are best to be examined while floating in some convenient transparent fluid. For instance, very few of the fibres of any of the muscles can be discovered when they are viewed in the open air; but if placed in water or oil, great part of their wonderful fabric may be discovered. If the thread of a ligament be viewed in this manner, it will be seen to consist of an indefinite number of smooth round threads lying close together.

Objects of an elastic nature should be pulled or stretched out while they are under the microscope, that the texture and nature of those parts, whose figure is altered by being thus pulled out, may be more fully discovered.

To examine BONES with the microscope. These should first be viewed as opake objects; afterwards, by procuring thin sections, they should be looked at as if transparent. The sections should be cut in all directions, and be well washed and cleaned; a degree of maceration will be useful in some cases. Or the bones may be put in a clear fire till they are red hot, and then taken out; by these means the bony cells will appear more conspicuous and visible, being freed from extraneous matter.

To examine the PORES OF THE SKIN. First, cut or pare off with a razor as thin a slice as possible of the upper skin; then cut a second from the same place; apply the last to the microscope.

The SCALES OF FISH should be soaked in water for a few days, and then be carefully rubbed, to clean them from the skin and dirt which may adhere to them.

To procure the scales of the eel, which are a great curiosity, and the more so, as the eel was not known to have any, till they were discovered by the microscope, take a piece of the skin of the eel that grows on the side, and while it is moist spread it on a piece of glass, that it may dry very smooth; when thus dried, the surface will appear all over dimpled or pitted by the scales, which lie under a sort of cuticle or thin skin; this skin may be raised with the sharp point of a penknife, together with the scales which will then easily slip out, and thus you may procure as many as you please.[43]

[43] Martin’s Micrographia Nova, p. 29.

On the lizard, the guana, &c. are two skins; one of these is very transparent, the other is thicker and more opake; by separating these we procure two beautiful objects.

The LEAVES of many trees, and some plants, when dissected, form a very pleasing object. To dissect them, take a few of the most perfect leaves you can find, and place them in a pan with clean water; let them remain three weeks or a month without changing the water, then take them up, and try if they feel very soft, and appear almost rotten; if so, they are sufficiently soaked. You are then to lay them on a flat board, and holding them by the stalk, draw the edge of the knife over the upper side of the leaf, which will take off most of the skin; turn the leaf, and do the same with the under side. When the skin is taken off on both sides, wash out the pulpy matter, and the fibres will be exhibited in a beautiful manner. By slitting the stalk you may separate the anatomized leaf into two parts. The skins that are peeled from the fibres will also make a very good object. The autumn is the best season for the foregoing operation, as the fibres of the leaves are much stronger at that season, and less liable to break.

ORES and MINERALS should all be carefully washed and cleansed with a small brush, to remove any extraneous matter that may adhere to them. SHELLS may be ground down on a hone, by which their internal structure will be displayed.

TO VIEW THE CIRCULATION AND EXAMINE THE PARTICLES OF THE BLOOD. The principal part the observer must aim at, in order to view the circulation of the blood, is to procure those small animals or insects that are most transparent, that by seeing through them he may be enabled to discover the internal motion. The particular kinds best adapted for the purpose will be enumerated in the descriptive catalogue at the end of this work.

If a small eel be used for this purpose, it must be cleansed from the slime which covers it; after which it maybe put either in the fish-pan, or a glass tube filled with water, and then placed under the microscope. If the eel be small enough, the circulation may be viewed in the most satisfactory manner. Leeuwenhoeck has given, in his 112th Epistle, an accurate description of the blood vessels in part of the tail of an eel. The same figure may also be seen in my father’s Micrographia Illustrata, fourth edition, Plate XVII. The tail of any other small fish may be applied in the same manner, or tied on a slip of flat glass, and be thus laid before the microscope. Flounders, eels, and gudgeons, are to be had at almost any time in London. N. B. By filling the tube with water, when an eel is used, it will in a great measure prevent the sliminess of the eel from soiling the glass.

To view the particles of the blood, take a small drop of it when warm, and spread it as thin as possible upon a flat piece of glass. By diluting it a little with warm water, some of the larger particles will divide from the smaller, and many of them will be subdivided into still smaller; or a little drop of blood may be put into a capillary tube of glass, and be then presented before the microscope. Mr. Baker advises the mixing the blood with a little warm milk, which he says, will cause the unbroken particles to be very distinctly seen. But the most accurate observer of these particles was Mr. Hewson, and he says they have been termed globules with great impropriety, being in reality flat bodies. When we consider how many ingenious persons have been employed in examining the blood with the best microscopes, it appears surprizing that the figure of the particles should be mistaken; but the wonder is lessened when we reflect how many obvious things are overlooked, till our attention is particularly directed towards them; and besides, the blood in the human subject, and in quadrupeds, is so full of these particles, that it is with great difficulty they can be seen separate, until the blood is diluted. It was by discovering a proper method to effect this, that Mr. Hewson was indebted for his success. He diluted the particles with serum, in which they would remain undissolved, and as he could dilute them to any degree with the serum, he could easily examine the particles distinct from each other; for example, take a small quantity of the serum of the human blood, and shake a piece of crassamentum in it, till it be coloured a little with the red particles; then with a soft hair pencil spread a little of it on a piece of thin glass, and place this glass under the microscope, in such a manner as not to be quite horizontal, but rather higher at one end than the other; by which means the serum will flow from the higher to the lower extremity, and as it flows, some of the particles will be found to swim on their flat sides, and will appear to have a dark spot in the middle; others will turn over from one side to the other, as they roll down the glass.

Several authors have described an apparatus for viewing the circulation of the blood in the mesentery of a frog; but as the cruelty attendant on these kinds of investigations would deprive the humane reader of a great part of the gratification which might otherwise result from them, he will probably rest satisfied with the accounts of such experiments to be met with in authors; especially as there is an abundant variety of objects on which he may exercise his ingenuity without sacrificing the nicer feelings of humanity.[44]

[44] Whatever right mankind may claim over the lives of every creature that is placed in a subordinate rank of being to themselves, in respect of food and self-defence, as well as for the improvement of science, and their judicious and ingenious application to the various purposes of use and ornament in human life, we certainly cannot, on the principles of reason and justice, assert a privilege to gratify a wanton curiosity, or the sports of an inordinate fancy, by the exercise of an unnecessary cruelty over them. The immortal SHAKSPEARE, in a passage which has often been quoted, says,

the poor beetle that we tread upon In corporal sufferance finds a pang as great As when a giant dies.

It may, however, be doubted whether this particular instance is strictly conformable to fact; different animals certainly possess different degrees of sensibility, and some are consequently more susceptible of pain than others. It is a remarkable circumstance that the Hippobosca equina, or Horse-fly, will live, run, nay even copulate, after being deprived of its head; most flies will survive that loss for some time, and the loss of a leg or two does not prevent their appearing as lively and alert as if they had sustained no injury. Many insects, on being caught, will freely and voluntarily part with their limbs to escape; and it is well known that lobsters shed their claws. Numbers of other instances might be adduced, but on this subject it may be prudent not to enlarge.

Montaigne remarks, that there is a certain claim of kindness and benevolence which every species of creatures has a right to, from us. It is to be regretted, that this general maxim is not more attended to in the affairs of education, and pressed home upon tender minds in its full extent and latitude; the early delight which children discover in tormenting different animals should by all possible means be discouraged, as, by being unrestrained in such sports, they may at least acquire a habit of confirmed inattention to every kind of suffering but their own, if not progressively be led to the perpetration of more atrocious acts of cruelty. The supreme court of judicature at Athens thought an instance of this sort not below its cognizance, and punished a boy for putting out the eyes of a poor bird that had unhappily fallen into his hands; and the inimitable HOGARTH, “the great painter of mankind,” has in his “Five Stages of Cruelty,” admirably depicted the consequences which may result from an early indulgence of a propensity towards cruelty.

In order to awaken as early as possible in the minds of children an extensive sense of humanity, it might be prudent to indulge them with a view of several sorts of insects as magnified by the microscope, and to explain to them that the same marks of divine wisdom prevail in the formation of the minutest insect, as in the most enormous leviathan; that they are equally furnished with whatever is necessary, not only for the preservation, but the happiness of their beings, in that class of existence which Providence has assigned them: in a word, that the whole construction of their respective organs distinctly and decisively, proclaims them the objects of divine benevolence, and therefore they justly ought to be so of ours. EDIT.

OF ANIMALCULA IN INFUSIONS, &C.